Composite
Part:BBa_K2957008:Design
Designed by: Melody Wu Group: iGEM19_MIT (2019-10-19)
CMV, IL-8 with NeonGreen Tag (C-terminal)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1802
Illegal SpeI site found at 92
Illegal PstI site found at 1245 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 92
Illegal PstI site found at 1245 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1019
Illegal BamHI site found at 1261 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1802
Illegal SpeI site found at 92
Illegal PstI site found at 1245 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1802
Illegal SpeI site found at 92
Illegal PstI site found at 1245
Illegal AgeI site found at 1808 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used Modular Cloning Golden Gate Type II Assembly, so we had to consider how parts came together. We also used the natural secretion tag given in the part so we could use it to test chemokine secretion success by HEKs. We also had to consider which terminal the fluorescence/protein tag was added so as not to render the chemokine dysfunctional. See more on the MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT>.
Source
Parts of this part were ordered from IDT/Twist and then assembled with other parts (from iGEM/parts in our parts collection/from the Weiss lab) into this plasmid.